green multiplex master mix (New England Biolabs)

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New England Biolabs green multiplex master mix
Green Multiplex Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 361 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiplex+pcr+master+mix/Multiplex+PCR+Master+Mix/pm41981313-338-16-20
Average 97 stars, based on 361 article reviews

green multiplex master mix - by Bioz Stars, 2026-09

97/100 stars

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Article Title: Seedborne<i>Cercospora beticola</i>Can Initiate Cercospora Leaf Spot from Sugar Beet (<i>Beta vulgaris</i>) Fruit Tissue
Article Snippet: Cercospora leaf spot (CLS) is a globally important disease of sugar beet (Beta vulgaris) caused by the fungus Cercospora beticola.. Longdistance movement of C. beticola has been indirectly evidenced in recent population genetic studies, suggesting potential dispersal via seed.. Commercial sugar beet “seed” consists of the reproductive fruit (true seed surrounded by maternal pericarp tissue) coated in artificial pellet material.

Article Title: High-throughput and Efficient Assay for Central Nervous System Infection with Targeted Nanopore Sequencing Technology
Article Snippet: Sanger sequencing was performed using an ABI PRISM 3730 DNA Sequencer (Applied Biosystems, Foster, CA, USA), which was run by Shenggong Biotechnology (Shanghai, China). .. Multiplex PCR of tNPS The 25 μL multiplex PCR master mix contained 1× Q5 Hot-Start HiFi Buffer (New England Biolabs, Ipswich, MA, USA), 200 μM dNTPs, 0.2 μM each primer, 1× High GC enhancer, 0.5 U Q5 Hot-Start High-Fidelity DNA Polymerase (New England Biolabs), and 10 μL template DNA. .. Thermal cycling parameters were optimized as follows: initial denaturation at 98°C for 1 min; 35 cycles of denaturation at 95°C for 25 s; annealing at 57°C for 30 s; extension at 72°C for 3 min; and final extension at 72°C for 4 min. To minimize primer interference, amplification was partitioned into two reaction panels: (1) bacterial-fungal panel: simultaneous detection of seven bacterial pathogens (S. pneumoniae, N. meningitidis, E. coli, L. monocytogenes, M. tuberculosis, H. influenzae and S. agalactiae) and one fungus (C. neoformans); and (2) viral panel, covering nine neurotropic DNA viruses (HSV-1, HSV-2, VZV, CMV, HHV-6, HHV-7, AdV, EBV, and JCV).

Article Title: High-throughput and Efficient Assay for Central Nervous System Infection with Targeted Nanopore Sequencing Technology
Article Snippet: Sanger sequencing was performed using an ABI PRISM 3730 DNA Sequencer (Applied Biosystems, Foster, CA, USA), which was run by Shenggong Biotechnology (Shanghai, China). .. The 25 μL multiplex PCR master mix contained 1× Q5 Hot-Start HiFi Buffer (New England Biolabs, Ipswich, MA, USA), 200 μM dNTPs, 0.2 μM each primer, 1× High GC enhancer, 0.5 U Q5 Hot-Start High-Fidelity DNA Polymerase (New England Biolabs), and 10 μL template DNA. .. Thermal cycling parameters were optimized as follows: initial denaturation at 98°C for 1 min; 35 cycles of denaturation at 95°C for 25 s; annealing at 57°C for 30 s; extension at 72°C for 3 min; and final extension at 72°C for 4 min. To minimize primer interference, amplification was partitioned into two reaction panels: (1) bacterial-fungal panel: simultaneous detection of seven bacterial pathogens ( S. pneumoniae , N. meningitidis, E. coli, L. monocytogenes, M. tuberculosis, H. influenzae and S. agalactiae ) and one fungus ( C. neoformans ); and (2) viral panel, covering nine neurotropic DNA viruses (HSV-1, HSV-2, VZV, CMV, HHV-6, HHV-7, AdV, EBV, and JCV).

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Article Title: High-throughput and Efficient Assay for Central Nervous System Infection with Targeted Nanopore Sequencing Technology
Article Snippet: Sanger sequencing was performed using an ABI PRISM 3730 DNA Sequencer (Applied Biosystems, Foster, CA, USA), which was run by Shenggong Biotechnology (Shanghai, China). .. The 25 μL multiplex PCR master mix contained 1× Q5 Hot-Start HiFi Buffer (New England Biolabs, Ipswich, MA, USA), 200 μM dNTPs, 0.2 μM each primer, 1× High GC enhancer, 0.5 U Q5 Hot-Start High-Fidelity DNA Polymerase (New England Biolabs), and 10 μL template DNA. .. Thermal cycling parameters were optimized as follows: initial denaturation at 98°C for 1 min; 35 cycles of denaturation at 95°C for 25 s; annealing at 57°C for 30 s; extension at 72°C for 3 min; and final extension at 72°C for 4 min. To minimize primer interference, amplification was partitioned into two reaction panels: (1) bacterial-fungal panel: simultaneous detection of seven bacterial pathogens ( S. pneumoniae , N. meningitidis, E. coli, L. monocytogenes, M. tuberculosis, H. influenzae and S. agalactiae ) and one fungus ( C. neoformans ); and (2) viral panel, covering nine neurotropic DNA viruses (HSV-1, HSV-2, VZV, CMV, HHV-6, HHV-7, AdV, EBV, and JCV).

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